gfp trap a agarose beads (Proteintech)

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Proteintech gfp trap a agarose beads
Gfp Trap A Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+trap+agarose+beads/TRAPA%2FSSR1+Antibody/pm41115472-174-26-29
Average 96 stars, based on 693 article reviews

gfp trap a agarose beads - by Bioz Stars, 2026-11

96/100 stars

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Immunoprecipitation:

Article Title: TUG protein acts through a disordered region to organize the early secretory pathway.
Article Snippet: Post fixation cells were blocked in PHEM buffer 5% normal goat serum for at least 30min and stained using antibodies against LC3A/B (Cell Signaling Technology, Cat. No. 12741S) and PDI (Enzo Life Sciences, clone 1D3, Cat. No. ADISPA-891). .. The following day, cells were immunoprecipitated using GFP trap agarose beads (Proteintech; gta) according to themanufacturer’s protocols. .. Cells were then imaged on Zeiss 880 using ×63/1.4 oil objective at room temperature.

Article Title: The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A.
Article Snippet: Article

Article Title: TUG protein acts through a disordered region to organize the early secretory pathway
Article Snippet: Cells were then imaged on Zeiss 880 using ×63/1.4 oil objective at room temperature. .. The following day, cells were immunoprecipitated using GFP trap agarose beads (Proteintech; gta) according to the manufacturer’s protocols. ..

Incubation:

Article Title: Abscisic acid‐, stress‐, ripening‐induced 2 like protein, TaASR2L , promotes wheat resistance to stripe rust
Article Snippet: Two or three days after infiltration, the leaves were collected for protein extraction using lysis buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 0.5% wt/vol NP40, 0.1% vol/vol Triton X‐100, 1 mM PMSF, and 1× proteinase inhibitor [Solarbio]). .. Samples were centrifuged to retain the supernatant and incubated at 4°C for 3 h with GFP‐trap agarose beads (ChromoTek) in a 2 mL tube (Yang et al., ). .. After washing with wash buffer, the immunoprecipitated proteins were boiled in 2 × SDS sample buffer (Beyotime).

Article Title: ATG8ylation of vacuolar membrane protects plants against cell wall damage
Article Snippet: After the first centrifugation, the supernatant was filtered with Miracloth (Sigma-Aldrich). .. The supernatant was incubated with 30 μl GFP-Trap ® Agarose beads (Chromotek) for 1,5 h. Beads were washed three times with Wash Buffer (10% glycerol, 10 mM DTT, Tris pH 7.4, 50 mM KCl, 2mM MgCl2, 1 mM ATP, 0.1% dodecyl beta-D-maltoside, 20 μg/mL Pepstatin, 1 tablet/50 mL cOmplete EDTA-free Protease Inhibitor Cocktail [Roche]) before and after incubation with lysate. ..

Article Title: The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A.
Article Snippet: Article

Protease Inhibitor:

Lysis:

Article Title: ATG9 vesicles are a subtype of intracellular nanovesicle
Article Snippet: .. During lysis, GFP-Trap Agarose beads (chromotek, gta-20), 25 μL per reaction, were prepared by washing three times with 500 mL INV release buffer with spins at 2500 g for 2 min at 4 °C before final resuspension in INV release buffer (50 μL per reaction). ..

Mass Spectrometry:

Article Title: A fungal ubiquitin ligase and arrestin binding partner contribute to pathogenesis and survival during cellular stress
Article Snippet: .. Immunoprecipitations from normalized total cell lysates were performed by the addition of 25 μL GFP-TRAP agarose beads (ChromoTek, Proteintech, Germany) and inversion at 4°C for 2 h. Mass spectrometry analysis was performed on immunoprecipitations by the Duke Proteomics and Metabolomics Core Facility, as described previously ( ). .. Immunoprecipitations from normalized total cell lysates were performed by the addition of 25 μL GFP-TRAP agarose beads (ChromoTek, Proteintech, Germany) and inversion at 4°C for 2 h. Mass spectrometry analysis was performed on immunoprecipitations by the Duke Proteomics and Metabolomics Core Facility, as described previously ( ).

Article Title: A fungal ubiquitin ligase and arrestin binding partner contribute to pathogenesis and survival during cellular stress.
Article Snippet: .. Immunoprecipitations from normalized total cell lysates were performed by the addition of 25 μL GFP-TRAP agarose beads (ChromoTek, Proteintech, Germany) and inversion at 4°C for 2 h. Mass spectrometry analysis was performed on immunoprecipitations by the Duke Proteomics and Metabolomics Core Facility, as described previously (28). .. The resinbound immunoprecipitated proteins were treated with 1% RapiGest (Waters) at 50°C for 10 minutes, reduced by the addition of 10 mM dithiothreitol and incubation at 32°C for 35 minutes, and finally alkylated by the addition of 20 mM final concentration iodoa cetamide and incubation at room temperature for 30 minutes.

Recombinant:

Article Title: The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A.
Article Snippet: Article

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